Abstract: In previous paper ( 2019 Int. J. Mod. Phys. B 33 1950197 ; 2020 Int. J. Mod. Phys. B 34 2050022 ), we presented a method to judge the entanglement of 2-qubit system. The necessary and sufficient conditions for the 2qubit system being separable are that if the relevant coefficients is positive and the principal density matrix is separable, then the system is separable, otherwise it is entangled. Now in this paper, we try to generalize this criterion to a 3-qubit system, and then, we further generalize the criterion of 3-qubit system to an N-qubit system. This is a complicated and interesting issue.